LIF Protéine

N° du produit ABIN1589655

Cette protéine Recombinant LIF est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour LIF Protéine (ABIN1589655)

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Pureté: > 98 % by SDS-PAGE

Détail du produit

Fonction: LIF

Séquence: SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF

Specificité: Chromosomal location:22q12.2

Attributs du produit: Length (aa):180

niveau d'endotoxine: < 0.1 ng per μg (IEU/μg) of rh LIF

Information d'application

Indications d'application: The ED50 as determined by the M1 cell differentiation assay is in the range of 0.1 - 0.5 ng/mL.

Commentaires:

Cytokines & Growth Factors

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: The lyophilized LIF should be reconstituted in water to a concentration not less than 100 μg/mL. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.

Buffer: 50 mM acetic acid

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Lyophilized Leukemia Inhibitory Factor (LIF) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leukemia Inhibitory Factor (LIF) should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Détail du antigène

Antigène: LIF (Leukemia Inhibitory Factor (LIF))

Autre désignation: LIF

Sujet: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF. Recombinant human LIF produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 19.7 kDa.
Synonyms: LIF, CDF, DIA, HILDA, MLPLI

Poids moléculaire: 19.7 kDa

ID gène: 3976

NCBI Accession: NM_002309, NP_002300

UniProt: P15018

Pathways: Signalistation JAK/STAT, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding